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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
02/01/2017 |
Actualizado : |
15/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
CASTILLO, A.; GAIERO, P.; LÓPEZ CARRO, B.; VILARO, F. |
Afiliación : |
ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; P. GAIERO, Universidad de la República (UdelaR)/ Facultad de Agronomía; B. LÓPEZ CARRO, SECIF-IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Gametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
Plant Tissue Culture and Biotechnology, 2016, v. 26 (2): 159-173. |
DOI : |
10.3329/ptcb.v26i2.30566 |
Idioma : |
Inglés |
Contenido : |
ABSTRACT.
The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding. |
Palabras claves : |
ANTHER CULTURE; DOUBLE HAPLOID; FLOW CYTOMETRY; HAPLOID. |
Thesagro : |
GERMOPLASMA; PAPA; SOLANUM COMMERSONII. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13501/1/30566-Article-Text-109802-1-10-20161210.pdf
https://www.banglajol.info/index.php/PTCB/article/view/30566
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Marc : |
LEADER 01724naa a2200253 a 4500 001 1056407 005 2019-10-15 008 2016 bl uuuu u00u1 u #d 024 7 $a10.3329/ptcb.v26i2.30566$2DOI 100 1 $aCASTILLO, A. 245 $aGametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding.$h[electronic resource] 260 $c2016 520 $aABSTRACT. The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding. 650 $aGERMOPLASMA 650 $aPAPA 650 $aSOLANUM COMMERSONII 653 $aANTHER CULTURE 653 $aDOUBLE HAPLOID 653 $aFLOW CYTOMETRY 653 $aHAPLOID 700 1 $aGAIERO, P. 700 1 $aLÓPEZ CARRO, B. 700 1 $aVILARO, F. 773 $tPlant Tissue Culture and Biotechnology, 2016$gv. 26 (2): 159-173.
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Registro original : |
INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
21/02/2014 |
Actualizado : |
25/10/2016 |
Tipo de producción científica : |
Actividades de Difusión |
Autor : |
SAWCHIK, J.; [I.E. SAWCHIK, J.]; GUTIERREZ, F. |
Afiliación : |
JORGE SAWCHIK PINTOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE SAWCHIK PINTOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FELIX ALBERTO GUTIERREZ ZAMIT, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Leguminosas anuales para cultivos de cobertura ; parada no. 2 : campo experimental no. 1 Pasturas, INIA La Estanzuela. |
Fecha de publicación : |
2012 |
Fuente / Imprenta : |
ln: Día de Campo de Pasturas de INIA (2012, La Estanzuela, Colonia, UY) Gira pasturas 2012, zona Litoral Sur. La Estanzuela: INIA, 2012. |
Páginas : |
p. 5-7. |
Serie : |
(Serie Actividades de Difusión; 696) . |
Idioma : |
Español |
Palabras claves : |
AMOREIRAS; BARRIL; BINDOON; ESTANZUELA CIENTO DIECISÉIS; GERALLTON; GOULBURN; INIA CALIPSO; JOSÉ; LE NUEVE MIL TRESCIENTOS TREINTA Y TRES; LUPINUS LUTEUS; MEDICAGO LITORALIS; MEDICAGO SCUTELLATA SAVA; PARADARA; RIVERINA; SAGIT; TAIPÁN; TRAIGUÉN; TRÉBOL ALEJANDRINO; TRÉBOL BALANZA; TRÉBOL ENCARNADO; TRÉBOL MICHELANUM; TRÉBOL RESUPINATUM; TRÉBOL ROJO; TRÉBOL SQUARROSUM; TRÉBOL SUBTERRÁNEO; TRÉBOL VESICULOSUM; VICIA SATIVA; VICIA VILLOSA. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/665/1/112761161012094922.pdf
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Marc : |
LEADER 01478naa a2200493 a 4500 001 1048246 005 2016-10-25 008 2012 bl uuuu u00u1 u #d 100 1 $aSAWCHIK, J. 245 $aLeguminosas anuales para cultivos de cobertura ; parada no. 2$bcampo experimental no. 1 Pasturas, INIA La Estanzuela. 260 $c2012 300 $ap. 5-7. 490 $a(Serie Actividades de Difusión; 696) . 653 $aAMOREIRAS 653 $aBARRIL 653 $aBINDOON 653 $aESTANZUELA CIENTO DIECISÉIS 653 $aGERALLTON 653 $aGOULBURN 653 $aINIA CALIPSO 653 $aJOSÉ 653 $aLE NUEVE MIL TRESCIENTOS TREINTA Y TRES 653 $aLUPINUS LUTEUS 653 $aMEDICAGO LITORALIS 653 $aMEDICAGO SCUTELLATA SAVA 653 $aPARADARA 653 $aRIVERINA 653 $aSAGIT 653 $aTAIPÁN 653 $aTRAIGUÉN 653 $aTRÉBOL ALEJANDRINO 653 $aTRÉBOL BALANZA 653 $aTRÉBOL ENCARNADO 653 $aTRÉBOL MICHELANUM 653 $aTRÉBOL RESUPINATUM 653 $aTRÉBOL ROJO 653 $aTRÉBOL SQUARROSUM 653 $aTRÉBOL SUBTERRÁNEO 653 $aTRÉBOL VESICULOSUM 653 $aVICIA SATIVA 653 $aVICIA VILLOSA 700 1 $a[I.E. SAWCHIK, J.] 700 1 $aGUTIERREZ, F. 773 $tln: Día de Campo de Pasturas de INIA (2012, La Estanzuela, Colonia, UY) Gira pasturas 2012, zona Litoral Sur. La Estanzuela: INIA, 2012.
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INIA La Estanzuela (LE) |
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